I bought the Canon MP-E 65mm macro lens in February 2009 but not had a chance to properly try it out. Spurred on by a recent article in Amateur Photographer, I got the lens out & collected a few insects to try it out on.
The wasp was a dead example I found in the shed. It was placed on top of a small perspex box in the light of a window. Radio shutter release was used, I think the shutter speeds were around 1/2 a second.

For each of the images I took 20-40 photographs all taken at a slightly different focusing range. All the shots are then loaded into image stacking software – in this case Zerene Stacker – which goes off & takes the sharp part out of every image, combining them & deleting the rest. Thus you end up with a photograph with a much wider depth of field than is possible with the lens in a single shot.
Taking 40 shots of an insect is only possible if the subject is really still. In the case of the wasp, that was fine as it was dead. With the spider, I put it in the fridge for a couple of minutes. I’m told that no harm is done to the creature & it goes into a state of sleep as if in a cold night, though you do have to be careful not to cool it for too long.

The ant is in the state of waking up from its brief winter sleep. In fact on the second shot it was preening its antennae so I wasn’t able to get a series of shots to focus-stack. This is a single image, you can see how narrow the depth of field is with just one shot.
The woodlouse was a dead specimen found in the garden.

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Photo Shoots